英文名称: HK; Hexokinase from Saccharomyces cerevisiae; ATP: D-Hexose-6-phosphotransferase; Hexokinase from yeast
其他名称: 己糖磷酸激酶
CAS号: 9001-51-8

级别: BR

类型: Type F-300
分子量: ~ 54 kDa (monomer) ;~110 kDa (dimer)
活力: ≥130 units/ mg protein (biuret)

活力定义: One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25℃
Optimal pH: 7.5~9.0

Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm

Anion traces: sulfate (SO42-) : free

Foreign activity: ATPase, myokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase and phosphoglucose isomerase ≤0.01%
性状: 白色鳞状粉末. 酿酒酵母提取. 催化剂 (Activators) : Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds; 抑制剂 (Inhibitors) : sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)
用途: 生化研究. 为催化己糖6位上由ATP使之产生磷酸化反应的酶. 对α-D葡萄糖的亲和性很高 (Km=1*10-5M) ,对其他的己糖也可进行磷酸化. 此反应的平衡向右偏移. •G°'=4.0千卡. 该酶可被Mg2 , K 所活化, 但可被HS试剂阻抑.
保存: -20℃