英文名称: Luciferase firefly; Luciferase from Photinus pyralis (firefly)

其他名称: 荧光素酶 (萤火虫) ;重组型酵素

CAS号: 61970-00-1

级别: BR

product line: BioUltra

Assay: ≥98% (SDS-PAGE)

Mol wt: mol wt 120 kDa (two non-identical subunits, each containing four free thiol groups, one of which is necessary for activity)

Protein: 10-35% E1%/ 280

Measuring range: ≤1 femtomole ATP sensitivity. (using 0.2 μg of luciferase and suitably sensitive liquid scintillation counters or luminometers)

Foreign activity: ATPase ≤5 nanomolar units/ mg protein; Nucleoside diphosphokinase ≤20 nanomolar units/ mg protein

活力定义: One light unit produces a biometer peak height equivalent to 0.02 μCi of 14C in PPO/ POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25 °C

产品描述: Two contaminant, ATP-consuming activities are assayed for in this product, ATPase and nucleoside diphosphokinase. These impurities are found to be less than 5 nanomolar units/ mg protein and less than 20 nanomolar units/ mg protein, respectively.

Note: Prior to 1991, a unit of firefly luciferase activity was defined as that amount which will produce 1.0 nanomole of pyrophosphate per minute at pH 7.7, 25 °C, using a system containing 0.6 mM ATP and 0.1 mM D-luciferin. The former nanomolar unit is equivalent to approximately 1.3 x 106 light units

性状: 粉末.EC: 1.13.12.7

用途: 生化研究.

保存: -20℃. 保质期1年