英文名称: Protease S Aureus; Endoproteinase Glu-C from Staphylococcus aureus V8; V8 Protease

CAS号: 66676-43-5

级别: BR

来源: Staph aureus V8

Activity: ≥500units/ mg

活力定义: One Unit causes a change of 0.001 A280 nm per minute at 37℃, pH 7.8 using casein as the substrate

产品描述: Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol. , 45, 469, 1976) .

性状: 粉末.

用途: 生化研究.

保存: 2~8℃