BM-19AY trinocular emission fluorescence microscope, trinocular hinge 30 ° tilt tube can be free to rotate 360. Using the general fluorescence staining and immunofluorescence staining method can show the various components of cells, especially for cells (DNA and RNA) and acidic mucopolysaccharides have a strong specificity, so the fluorescent staining can be used as a good method of cytochemistry.Immunofluorescence method is the combination of immunological techniques and fluorescence staining of a It has the immunological specificity and the fluorescence method sensitivity, applies to the medicine, the pathological research, as well as the autoimmune disease diagnosis and so on, obtained the widespread application. two, Technical specifications1, eyepiece: | category | gain | Field Diameter (mm) | | Ultra Wide Field Field Eyepiece | 10X | Φ22 | | Wide field field field eyepieces | 16X (optional) | Φ18 |
2, the objective: | category | gain | The numerical aperture (NA) | system | Working distance (mm) | Infinity fluorescence Flat - field achromatic objective | 4X | 0.10 | dry | 37.5 | | 10X | 0.25 | 7.31 | | 40X | 0.65 | 0.63 | | 100X | 1.25 | oil | 0.18 |
3, magnification: | Objective | eyepiece | Tube coefficient | Total magnification | | 4X | 10X | 1X | 40X | | 10X | 100X | | 40X | 400X | | 100 | 1000X |
4, mechanical tube length: infinity system (∞) 5, tube lens: focal length f = 200mm 6, focusing device: 30 mm coarse coaxial focusing with focusing limit, coarse focus tension locking device 7, fine tune lattice value: 0.002mm 8, the stage: 180 × 150mm, moving range: 75 × 50mm, the standard grid of 0.1mm 9, the light source: drop lighting 220V (110V) / 100W mercury lamp, power box, transmission lighting: Halogen 12V / 30W. Kohler lighting, brightness adjustable. 10, excitation group: G, B, V, U band |