English name: Collagenase from Clostridium histolyticum; Clostridiopeptidase A
Other names: collagenase; collagenase; collagenase
CAS: 9001-12-1

Level: BR

Type: Ⅲ type
Source: dissolved organization prismatic Bacillus
Vitality: ≥125CDU / mg solid (CDU = collagen digestion units)
Activity was defined: One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at pH 7.5 at 25 ° C in the presence of calcium ions One neutral protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole tyrosine. per 5 hr at pH 7.5 at 37 ℃. One clostripain unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25 ℃ in the presence of DTT
Characters:. Tan or light brown powder optimum pH 7-8 with unique native collagen and denatured collagen digestion capacity detergents, HCH and heavy metal ions can reduce the activity of specific acts on the original. collagen to break and then by other proteolytic, not hydrolyzed fibrin, globulin
Use: Biochemical studies of mammalian tissue cell solution for off.
Save: -20 ℃