English name: Dnase Ⅰ; Deoxyribonuclease I (Bovine pancrease)
Other names: deoxy-cellular nucleases; oxygen RNA oligonucleotide 5'-hydrolase; RNase-free; DNA to polymerase; decarboxylase ribonuclease (bovine pancreatic); oxygen cellular nucleases (bovine pancreatic); DNA enzyme
CAS: 9003-98-9

Level: Refined
Type: Type IV
Molecular Weight: ~ 31 kDa
Vitality: ≥2000 Kunitz units / mg protein

Activity was defined:. One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 5.0 at 25 ℃, using DNA, Type I or III as substrate [Mg2 +] = 4.2 mM
Protein: ≥80%
Chymotrypsin: ≤0.5%
Protease: ≤0.05%
RNase: ≤0.02%
Characters: white or off-white lyophilized powder for the double-strand specific endonuclease, can degrade double-stranded DNA become oligodeoxyribonucleotide having 5'-phosphate and 3'-OH ends when there is a manganese ion. When the presence of single-stranded DNA can also degrade soluble in water, slightly soluble in 30% ethanol and acetone enzyme reaction: DNA → dinucleotide phosphate + 5' phosphate oligonucleotide 5' The optimum pH 7.8. absorptivity (E1% 280) 11.1. activator is a divalent metal ion, can be obtained from maximal activation of magnesium (Mg2 +) + calcium (Ca2 +). Inhibitors EDTA and sodium lauryl sulfate can be plus 5mmol / L calcium (Ca2 +)
Use: Biochemical Research Molecular Biology DNA of various enzymes for degrading enzymes sugar - phosphodiester bond hydrolysis phosphate backbone of these enzymes is divided into two types:... Exonuclease and within Many endonucleases .DNAase species, some single-stranded-specific, some specific double stranded, or sequence-specific (e.g., restriction endonucleases). the restriction endonuclease has a recognition sequence for a particular base capacity, this characteristic has been used in gene manipulation techniques to construct artificial clone.
Save: -20 ℃