English name : Alkaline proteinase
CAS: 9014-01-1
Molecular Weight: ~ 27300

Level : BR
Vitality : ≥200units / mg

Activity was defined : 1 g alkaline protease powder under the conditions pH1040 ℃, hydrolyzed casein per minute to produce 1 microgram of tyrosine, as an enzyme activity unit
Conditions of use: substrate concentration of 10 to 25%, temperature 50 ~ 60 ℃, PH value of 6.0 to 8.5, the reaction time is 3 to 6 hours (depending on the requirements can be long or short ), the reference amount of enzyme added 0.03 % to 0.06 % (by hydrolysis solution weight)
Properties: pale yellow powder or gray smooth particles are made ​​sterile by the Bacillus bacterial fermentation or by protoplast mutagenesis method B. subtilis 2709 made ​​sterile by deep microbial fermentation, extraction and refining of a proteolytic enzyme, belonging to a high alkaline serine protease crisp outside. the main components of subtilisin, is an enzyme within the catalytic site of serine
Use: Biochemical studies can hydrolyze protein molecules generated peptide or amino acid polypeptide with a strong ability to break down proteins.
Save : 2 ~ 8 ℃