English name: Hotstart Taq DNA Polymerase
Other names: Hotstart Taq DNA polymerase

Molecular weight: 94kDa, monomer

Level: BR

Purity: ≥99%
Concentration: 5u / ul
Activity definition: in 74 ℃, 30 minutes to allow the DNA synthesis to penetrate 10nmol polynucleotide (adsorbed on DE-81) the amount of enzyme
Activity assay mixture: 25mM TAPS (PH9.325 ℃), 50mM KC1, 2mM MgCL2, 0.2mM variety of dATP, dGTP, dTTP, 0.1mM dCTP, 0.75mM activated salmon sperm DNA and 0.4MBq / ML (3H). -dTTP
Save buffer components: 20mM Tris-HCL (PH9.0), 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.5% (V / V) Tween 200.5% (v / v) Nonidet P40 and 50% (v / v ) glycerin
10 * Hot start PCR Buffer: 200mM Tris-HCL (PH8.325 ℃), 200mM KC1, 50mM (NH4) 2SO4
Inhibitors: anionic detergent (deoxycholate, SDS and Sarkosyl concentration were higher than 0.060.02 and 0.01%)
Deactivation: phenol / chloroform extraction
Quality Control: Tests suggest that no endo or exo DNase, RNase contamination
Function Start: Hot Start PCR
Characters: Liquid designed to enhance the specificity, sensitivity and yield of DNA amplification can be formulated using the enzyme reaction system, easy to use at room temperature it is a chemically modified recombinant Taq DNA Polymerase, protein molecule amino acid residues. adds heat labile blocking groups. the enzyme inactive at room temperature, to avoid the formation of primer dimers and non-specific extension, thereby increasing the specificity of DNA amplification. 95 ℃ heated four minutes recoverable activity. activation Taq DNA Polymerase enzyme has the same function: 5-3 direction catalytic synthesis DNA, no detectable exonuclease activity correction 3-5, 5-3 has a lower exonuclease activity of the enzyme also has DNA. transfer enzyme activity in the 3 end of the PCR product to pay more 11 adenine, are not detected before activation activity.
Use: Biochemical studies of complex high yield amplification template; PCR specificity - to reduce the mismatch effect and reduces primer-dimer generated; enhanced PCR sensitivity; easy to use, prepared at room temperature PCR reaction system; amplification product has three -dA overhangs; hot start PCR; high yield amplification up to 3kb fragment; RT-PCR; specific amplification of complex genomic and cDNA templates; amplification of low copy DNA template; real-time PCR; multiplex PCR; for TA cloning PCR product
Save: -20 ℃, the shelf life of 1 year