English name : Protease S Aureus; Endoproteinase Glu-C from Staphylococcus aureus V8; V8 Protease

CAS: 66676-43-5

Level : BR

Source : Staph aureus V8

Activity: ≥500units / mg

Activity was defined : One Unit causes a change of 0.001 A280 nm per minute at 37 ℃, pH 7.8 using casein as the substrate

Product description :.. Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids In the presence of ammonium, the enzyme specificity is limited to glutamic sites has a molecular weight of 27000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol., 45, 469, 1976).

Characters : Powder .

Use: Biochemical research .

Save : 2 ~ 8 ℃